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Marine Sciences Division

Biotechnology Laboratories

The biotechnology laboratories are designed to facilitate research across in silico, bench, mesocosm, and ecosystem scales. The majority of the space is certified as Biosafety Level 2 (BSL2) workspace and is equipped for molecular environmental microbiology, microbial physiology, plant and animal tissue culture, genetic engineering and synthetic biology. With certified isolation lab spaces and other controlled spaces, we can work with invasive species and marine-toxin-producing organisms. We can also culture and manipulate viruses and autotrophic and heterotrophic prokaryotes, archaea, and microeukaryotes (including anaerobic, microaerophilic, and photosynthetic organisms).

These labs also have molecular biology capabilities, which are designed for processing tissue, water, laboratory, sediment, and other matrices with single cell/single molecule sensitivity. We have many genetic manipulation techniques to transform and transfect routine and non-traditional species, including biolistics and single cuvette through 96-well exponential decay and square wave electroporations. With a lab-on-a-chip bioanalyzer (Perkin Elmer/Caliper LabChip GXII), we can size profile and isolate DNA, RNA, protein, and glycans. We also have BSL2 ultracentrifugation available for purifying virus and separating subcellular features from prokaryotes and eukaryotes. Finally, our HPLC capabilities allow us to isolate and purify target molecules for downstream analysis.

Culturing bench

Culturing bench

Bioreactors

Bioreactors

Marine Sciences Laboratory

Research Areas

PNNL Websites

Contacts

Facilities

Class-100 Clean Laboratories

Trace Elemental and Chemical Speciation Analytical Capability

Mercury Analytical Laboratory

Organic Chemistry

Biotechnology Laboratories

Microalgal Research

Aquatics Research Laboratories

Hyperbaric Lab

Ice Lab

Oil Lab

Littoral Wave Tank

Biofouling/Biocorrosion Mesocosms

Climate-Simulated Aquaculture Raceway Ponds

Mineral Extraction from Seawater

Chem/Bio Isolation Laboratories

Electromagnetic Field Generators

Electronics/Optics Laboratories

Outdoor Research Platforms

Flow Tank

Greenhouse

Experimental Littoral Mesocosms

Wetland Research Sites

Research Vessels

Lidar Buoys

Scientific Dive Team

Littoral RDT&E Center